Pomegranate (Punica granatum L.) has been proved as having value in managing male sexual disorders. This purpose of this study is studiding the effect of pomegranate juice and peel on testosterone level and antioxidants activity in male rats.Minerals content, vitamins , fractions of polyphenols and were determined. The results showed that the pomegranate peel and juice had contained the highest amounts of Minerals content, vitamins and natural antioxidant compounds. Thirty-six adult male rats were divided into six groups, with six rats each group. Experimental rats were fed on basal diet for 15 days and randomly divided into six groups six rats for each. The 1st main group was fed on basal diet for another 6 weeks and considered as control negative rats .The other five groups administered 7 ml /kg cisplatin / kg. “positive control" the third group fed on basal diet and Served as (0.25 ml pomegranate juice/ rat) by oral tube.The fourth group fed on basal diet and received oral administration of 0.75 mL pomegranate juice /rat. The fifth group fed on basal diet and received 10 gm powder pomegranate peel /rat. the final group fed on basal diet and received oral administration of 20 gm powder pomegranate peel. At the end of biological experiential (five weeks) the gain body weight was recorded every three days for five week. Moreover, the blood samples were taken with drawn from the orbital plexus and centrifuged at 3000 rpm to obtain the sera after that, the sera were kept in a deep - freezer at -20°C until their analysis. Tests were dissected out and quickly frozen in liquid N2 for histological examination. The tissue specimens from tests were collected from treated and control rats by the end of the experiment. The results showed that the pomegranate juice contained P, Mn, Fe, and Cu at the level of 7.82, 5.12, 3.37, and 0.71 mg/100g dry matter respectively. The results observed that the pomegranate peel powder contained B1 (thiamine), B2 (riboflavin), C (L-ascorbic acid), E (α-Tochoferol) and A (Retinol) at the levels of 0.131, 0.11, 13.4, 3.25 and 0.15 mg/100g dry matter, versus 1.42, 0.97, 15.13, 1.79 and 0.09 mg/100g dry matter for pomegranate juice, respectively. The results indicated that the antioxidant enzymes GSH, G-PX, and CAT were the lowest in the cisplatin group (2) (14.08 mmol/l, 9.58 mmol/l, and 9.09 u/mg, respectively) which injection with cisplatin and fed on basal diet than other groups. Meanwhile, the control healthy group the highest in the antioxidant enzymes was 96.88 mmol/l, 79.11 mmol/l, and 12.20 u/mg, respectively, and fed on basal diet. The different cisplatin groups treated with pomegranate juice and peel powder had the best results thus show the rat's group (4) which taken orally one milliliter daily contained 0.50 mL pomegranate juice plus 0.50 mL distilled water and fed on a basal diet gave 43.63 mmol/l, 37.31 mmol/l and 9.72 u/mg, respectively. Moreover, the results from the group (6) which fed on basal diet substituted with 20g pomegranate peel give 58.59 mmol/l, 67.16 mmol/l, and 10.58 u/mg, respectively the best results when the rats fed on a high amounts and orally from pomegranate peel and juice and give the best results .In addition ,the results it could be noticed that all parameters especially fertility improvements and give the best results. Moreover, the histological examination of testis was confirmed .It could be noticed that the pomegranate juice and the peel were improvement all parameters especially fertility and sexual tract. Therefore, it could be recommended that pomegranate juice and the peel is a good source for health sexual.