Background: In recent years, phytochemicals have gained international scientific recognition for their ability to prevent major health problems. Research on the chemical structures and pharmacological mechanisms of plant extracts with anticancer effects has attracted a lot of attention. Work's objective: The work of the current study aimed to assess the chemotherapeutic anti-tumor ability of Tribulus terrestris extract against mammary carcinogenesis in Swiss albino mice produced by Ehrlich ascites carcinoma (EAC) implantation. Materials and methods: Tribulus terrestris extracts were evaluated for their effectiveness against EAC by tracking tumor weight, volume, and occurrence. Lipid peroxidation (MDA) and the biochemical oxidative stress-related profile were studied, as were the antioxidants-related profile, which included the activity of superoxide dismutase (SOD), glutathione reductase (GR), glutathione-s-transferase (GST), total antioxidant capacity (TAC), catalase, and CAT, as well as the hepatic and renal toxicity markers (creatinine, urea, and transaminases) and histopathological changes after treatment. The Results: showed that oxidative stress was reduced, antioxidant levels were raised, liver and kidney functions were restored, and tumor and neovascularisation were inhibited. Conclusion: Based on all obtained data, our research suggests that Tribulus terrestris extract treatment offered robust chemotherapeutic action as well as antioxidant defense against EAC-implanted mammary tumors.