This study aims to investigate the effectiveness of lipase and protease enzymes in removing soot stains from the paint layers of historical oil paintings while minimizing changes to color and chemical composition. Using experimental mock-ups based on a historical oil painting from Fatima Ismail Palace, varying concentrations (1%, 3%, and 5%) of each enzyme was applied. The results were evaluated using scanning electron microscope (SEM-EDX), colorimetric analysis, and Fourier transform infrared (FTIR) spectroscopy. The SEM analysis revealed that higher concentrations of both enzymes achieved greater stain removal. Colorimetric analysis indicated that 3% concentrations of both lipase and protease provided optimal cleaning with minimal color alteration, while higher concentrations led to increased color changes. FTIR analysis showed that the enzymes, particularly at 3% and 5%, altered specific chemical bonds associated with the soot stains. Overall, the study demonstrates the potential of lipase and protease enzymes in controlled cleaning of oil paintings, suggesting that 5% concentrations are most effective for removing stains while preserving the original paint layer's integrity.