This study was conducted to investigate the effects of fortifying biscuits with Milk Thistle seeds (MTS) on steatohepatitis disease in rats. Thirty-five rats were separated into two groups of seven each. The first group was fed a basal diet and kept as the negative control group, while the other rats were fed a basal diet deficient in Methionine and Choline with some modifications, including the addition of 19% fat and 1% soybean oil, for six weeks after the induction of steatohepatitis. Rats were reclassified into four equal groups as follows: subgroup 1 served as the control positive group, and the other three treated rat subgroups were fed a high-fat diet containing 100g of biscuit fortified with 5%, 10%, and 15% MTS, respectively. Results showed that biscuits with a 5-15% addition of milk thistle were of good quality. The enrichment of flour with milk thistle led to a significant increase in the essential nutrients, protein, and fat content of the biscuit. Despite the increased fat content, the caloric value of the milk thistle cookies decreased compared to the control group. The results showed that biscuits fortified with different levels of MTS improved the body weight and liver weight, accompanied by a significant decrease in liver function levels (ALT, AST, and Total bilirubin), as well as in lipid profile. There was also a significant increase in high-density lipoprotein-cholesterol (HDL-C). Additionally, Malondialdehyde (MDA) was significantly reduced, while the antioxidant enzymes (SOD and CAT) were significantly increased (P<0.05). In conclusion, biscuits fortified with MTS could serve as a natural therapy against steatohepatitis in rats.