Two field trials were carried out at Mattana Agricultural Research Station (Qena Governorate) in two growing seasons 1993/1994 and 1994/1995 to study the effect of phosphorus fertilizer and phospho­bacterin (biofertilizer) on growth, juice quality and yield of sugar 'cane. The trial consisted of twenty-four treatments which were the combina­tions between six levels of phosphorus fertilization (0, 15, 30, 45, 60 and 75 kg P₂05/fed.) which were allocated at random in the main plots and four levels of phosphobacterin (0, 250, 500 and 750 g Pb/fed.) which were randomly distributed in the sub plots. A split plot design with four replications was used. The plot size was 35m². Variety G. 85/37 was planted. The obtained results indicated that phosphorus levels had a sig­nificant influence on purity percentage while their effect on the length and diameter of stalks, sucrose percentage, cane and sugar yields were insignificant. The best level of phosphorus fertilization was 60 kg P₂051 fed. phosphobacterin levels significantly affected stalk length, sucrose percentage, cane and sugar yields. Mostly, applying 500 g Pb/fed gave the highest value for the previous characters. The interaction between phosphorus and phosphobacterin levels did not significantly affect all the studied attributes.