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363176

Genetic Identification and Phylogeny of Clerodendrum Phlomidis Growing in Egypt Using Some DNA Barcodes

Article

Last updated: 30 Dec 2024

Subjects

-

Tags

Genetics and Biotechnology

Abstract

Clerodendrum is one of the Lamiaceae genera, which has significance in taxonomical position. Clerodendrum phlomidis is an important wild species in Egypt and plays a main role in ecological, biodiversity, and taxonomical approaches. In the 
current study, we conducted this research work to investigate the identification of C. phlomidis collected from the North Coastal region of Egypt using three DNA barcode sequences viz., rbcL, ITS, and rpoC1 genes. The investigation was conducted in the corporation between the Agric. Botany-Genetics Dept., Fac. of Agric, Al-Azhar Univ. and National Gene Bank (NGB), Agricultural Research Center (ARC), Egypt. Our findings revealed that accessions KF199889 (98.7%) and ON010669.1 (98.6%) were identical to the taxon of C. phlomidis by the ITS and rbcL sequences. On the other hand, the sequence of C. phlomidis revealed closer to the taxa of C. buchneri, C. cephalanthum, C. thomsoniae, and C. rotundifoliuma by ITS sequences, C. indicum, C. thomsoniae, C. longiflorum, C. infortunatum, C. floribundum, C. cephalanthum, C. buchneri, and C. splendens by rbcL, and C. japonicum, C. thomsoniae, C. cyrtophyllum, and C. trichotomum by rpoC1 sequences. It can be recommended that these partial sequences are accurate genes for 
identifying and verifying the species of Clerodendrum phlomidis. 

DOI

10.21608/sjas.2024.287655.1421

Keywords

Clerodendrum phlomidis, ITS, rbcL, Molecular identification, blast

Authors

First Name

A.A

Last Name

Ateto

MiddleName

-

Affiliation

Agricultural Botany (Genetics) Department, Faculty of Agriculture, Al-Azhar University, Cairo11884, Egypt.

Email

agrigen91@azhar.edu.eg

City

-

Orcid

-

First Name

A.I.H

Last Name

Sayed

MiddleName

-

Affiliation

Agricultural Botany (Genetics) Department, Faculty of Agriculture, Al-Azhar University, Cairo11884, Egypt.

Email

-

City

-

Orcid

-

First Name

H.F

Last Name

EL-Shaer

MiddleName

-

Affiliation

Agricultural Botany (Genetics) Department, Faculty of Agriculture, Al-Azhar University, Cairo11884, Egypt.

Email

-

City

-

Orcid

-

First Name

E.M.B

Last Name

Mahdy

MiddleName

-

Affiliation

National Gene Bank (NGB), Agricultural Research Center (ARC), 9 Gamma St., 12619, Giza, Egypt.

Email

-

City

-

Orcid

-

Volume

6

Article Issue

2

Related Issue

48040

Issue Date

2024-06-01

Receive Date

2024-05-06

Publish Date

2024-06-01

Page Start

110

Page End

122

Print ISSN

2535-1796

Online ISSN

2535-180X

Link

https://sjas.journals.ekb.eg/article_363176.html

Detail API

https://sjas.journals.ekb.eg/service?article_code=363176

Order

363,176

Type

Original Article

Type Code

916

Publication Type

Journal

Publication Title

Scientific Journal of Agricultural Sciences

Publication Link

https://sjas.journals.ekb.eg/

MainTitle

Genetic Identification and Phylogeny of Clerodendrum Phlomidis Growing in Egypt Using Some DNA Barcodes

Details

Type

Article

Created At

30 Dec 2024