An experiment was carried out at the Experimental Station of Faculty Cairo University during two winter seasons of 2020/2021 and 2021/2022 to study the effect of foliar spray of Ca, Mg and Zn fertilizers in nanoparticles applied alone or in mixed composition on growth , yield, total soluble solids, changes in leaves anatomy structure biochemical components, enzymatic activity. The concentration of used fertilizers was 50 ppm which foliar sprayed cauliflowers compared to control plants that sprayed by water. Results indicated that the calcium fertilizer induced highest values of 
growth, yield, chlorophyll content, total soluble solids, leaf anatomical structure of relative to control. In addition to calcium nano-fertilizer had a significant effect on chlorophyll concentration and Chl. a/b. All nano-fertilizers had significant reduction on total soluble phenol compared to control. zinc nanoparticle recorded the highest significant total free amino acids as well as free proline accumulation. calcium nanoparticles showed the highest significant specific activity for both catalase as well as peroxidase in cauliflower plant.