A significant legume crop known for its high levels of protein and edible oil is the soybean. High levels of nitrogen are needed by soybean plants, and this nitrogen is primarily supplied by biological nitrogen fixation. A field test was performed by using soybean (Glycine max) Giza 82 cultivar.Growing soybean with or without Rhizobium spp. and Colchicine compared to non-inoculated plants, led to raise in some morphological features similar to this, infected 
plants displayed increased enzyme activity. Such as catalase, polyphenol, peroxidase and ascorbate oxidase,as compared to soybean plants that weren't inoculated . Colchicine concentration was the most crucial source of diversity across all traits. Significant responses were detected for various morphological parameters, anatomical features of root and leaf, and DNA content in the genotype used.High polymorphic bands were found in ISSR tested primers, and fingerprinting revealed 126 distinct DNA fragments in all has 105 fragments on average each primer. There were between one and three polymorphic bands per primer, with an average of 0.75.