El-Gharbia main drain poses a significant environmental threat, as it receives wastewater from three sources: sewage, agriculture, and industry. The objective of this study is to evaluate the impact of El-Gharbia main drain wastewater on the micronutrient and heavy metal content of surrounding soil and plant. Samples (soils and plants) were taken from fifteen sites along the drain in winter of 2020 and summer of 2021. Results showed that the content of soils and plants samples from micronutrients such as Fe, Mn, Zn, Cu and B and heavy metals such as Cd, Co, Cr, Ni and Pb) were differed from site to another, in some cases, the overall mean exceeded the permissible limit in both seasons, but the pollution degree was higher in the summer season. As for the bioaccumulation factor values (BCF) in plant samples were higher than 1. Application of risk assessment equations showed that the enrichment factor (EF) of micronutrients and heavy metals values were low level except for cadmium element values which varied from very significant to severe level and lead element values were moderate level in both seasons under study, contamination factor (CF) values were varied from low to moderate except for cadmium element were severe contamination level in both seasons, contamination degree (Cd) were varied from considerable to very high level in both seasons, pollution load index (PLI) values were high contamination level in both seasons except for site No. 1was low level at winter season.