Treating the liquids of the organic animal urine ‎loaded with microbial materials is highly significant. So, ‎employing bio-activated carbon (AC) for rabbit urine purification is a ‎major factor in avoiding and spreading diseases. The rabbit urine may be used ‎after treating as a good bio-fertilizer. Therefore, this research aims to identify‎ the best ‎components for bio-filter to purify and treat rabbit urine can used. ‎The investigated bio-filter components were: I- Two layers of ‎filter paper + one layer of “AC" with 5.0 cm height‎, II- Two ‎layers of filter paper + one layer of AC with 5.0 cm height‎ ‎between two layers of sand with 4.0 cm height, and III- Two ‎layers of filter paper + one layer of cork with 1.5 cm height‎ + ‎one layer of AC with 5.0 cm height + one layer of cork with 1.5 ‎cm height‎ + one layer of sand with 4.0 cm height + one layer of ‎cork with 1.5 cm height‎. The measurements were conducted to ‎determine the quality of the liquid produced as standard ‎fertilizer under different treatments in three replications. The ‎results indicated that the possibility of applying AC, which was made‎ ‎from date palm kernels, as a bio-filter media. This bio-filter was ‎acceptable with all the tested treatments due to the‎ ability to ‎purify impurities such as; aspect (light to clear), sedimentation, ‎pH (5.0 to 6.0), pus cells (3.0 to 6.0), red blood cells (1.0 to ‎‎4.0), organisms (non), and colony count (non)‎.