Galleria mellonella L. (Lepidoptera: Pyralidae) is known the main and the most harmful pest of beeswax all over the world. Propolis is a resinous substance collected by honeybees. It contains bioactive compounds that function for a variety of biological purposes. the aim of this study to controlling greater wax moth G. mellonella and restore colony activity by Using Propylene glycol extract of Moroccan propolis during from September to December 2023 in laboratory and apiary. The results obtained showed that the highest concentration of propolis extract was 1.4 ppm. It has recorded99.9 % of mortality percentage on larvae G. mellonellaafter 24hour. LC₅₀ value of propolis extract was recorded (0.440). All different bee activities (area of workers ​​brood, area of ​​stored pollen and area of ​​sealed honey) were shown to be improved or increased in different colonies after twelve days of treatment, meaning that the glycoprotein extract of propolis was effective in controlling wax worms and restoring the colony activity again. The using of propolis in controlling GWM may not cause subsequent damage to honeybee products such as wax and honey because it has no toxic residues, and it is a natural component of the bee colony.