Morphological characteristics of honey bee worker are important traits for breeding systems and useful for discriminating subspecies and ecotypes. During the season 2023, the present investigation took place at the Faculty of Agriculture at Al-Azhar University. Bees were collected from apiaries in the Assiut governorate region of Egypt to determine some Morphometrical characteristics proboscis length, Flagillum length, Total length of antenna, head width length, forewing length and width, hind wing length and width, numbers of hamuli,Cubital index, Femur length, Tibia length hand width, basitarsus length and width, hind leg length, first wax gland length and width, third sternum length and width. All bee samples were placed into a –20° C freezer at the laboratory until it was dissected for separation (Proboscis, forewing, hind wing, and hin dleg).The results showed that the mean values were: Proboscis length 5.69 ± 0.010 mm.; forewing length of 8.46 ± 0.019 mm. and width 2.93 ± 0.012 mm.; cubital index 2.29± 0.028 mm ; numbers of hamuli on hind wing 22.33± 0.16; first wax-gland length 1.35± 0.006 mm. and width 2.12 ± 0.016 mm. Hybrid bees can be distinguished by this character if they have Egyptian ancestry. The Egyptian honeybee in Assiut can be considered slightly non pure race.