Among vineyard management objectives is enhancing grapevine nutrition to control yield and berries quality. Experiments were carried out on the "Ruby seedless" (King Ruby) grape cultivar in pomology department's experimental orchard of the faculty of agriculture, Assiut University, during the two consecutive seasons of 2022 and 2023. This study sought to determine how certain plant-based natural oils affected the yield and quality of berries produced. Findings indicated that all utilized oils had a significant impact on physical and chemical characteristics When compared to control treatment the two studied seasons. Flax and bitter Almond seed oils at 1 and 2 ml./L resulted in enhancing almost physical properties. Black Cumin and Rocket plant seed oils greatly affected almost all characteristics. So, it can be recommended to spray these oils two weeks after the setting stage and at the beginning of coloring to improve the natural characteristics and quality of the fruits of the Ruby Seedless grape variety (King Ruby) under Assiut conditions.