At the Agronomy Department, Faculty of Agriculture, Mansoura University, Egypt, a laboratory experiment was carried out in March 2023 to investigate the impact of some artificial and natural substances to improve germination and seedling parameters of sugar beet Heba cultivar under various irrigation water sources. The investigation was carried out using factorial complete randomized design (CRD). The first factor included three different irrigation water sources (irrigation water, usual agricultural wastewater and industrial wastewater). The second factor comprised eight different methods of soaking seeds: without soaking “control", soaking in distilled water, boron (B), humic acid (HA), yeast extract (YE), silicon (Si), potassium (K), and macro and micro elements and amino acids (Macro + Micro + AA). Irrigation water was the control treatment that produced the greatest results in terms of germination and seedling characteristics. Usual agricultural wastewater came in second. Sugar beet seeds soaked in YE at a rate of 150 ml per liter produced the highest germination percentage, speed germination index, germination index, mean germination time, energy of germination, lengths of the plumel, radical, and seedlings, as well as the highest values of total chlorophyll, radical dry weight, and plumel dry weight and lowest values of abnormal and hard seed percentages. It may be advised to soak seeds for 18 hours in YE at a rate of 150 ml/L and irrigation by usual agricultural wastewater to improve sugar beet Heba cultivar germination and seedlings parameters.