Bio-stimulants have obtained global attention due to their beneficial impacts on the growth and development of several of crops. The current experiment was carried out during two winter seasons to assess the impact of different bio-stimulant substances on plant vigor, yield, and quality attributes in two strawberry cultivars (Fortuna and Festival) in a plastic greenhouse. Our treatments were: 1) humic acid 15% (10 ml L-1); 2) fulvic acid 15% (10 ml L-1); 3) potassium citrate (2 g L-1); 4) potassium silicate (2 g L-1); 5) salicylic acid (1 g L-1); 6) calcium carbonate (2 g L-1); and 7) control (water). Strawberry plants were treated weekly with each bio-stimulant, started after 15 days of transplanting and repeated 10 times. The most effective bio-stimulants were calcium carbonate and potassium silicate. Calcium carbonate enhanced all studied traits except dry matter, alkaloids concentration, antioxidant activity in leaves, and titratable acidity, while potassium silicate improved all traits except dry matter and alkaloids in leaves. Potassium citrate showed the highest value of flavonoids in leaves, firmness, and TSS. Humic acid exhibited the highest antioxidant activity in leaves and titratable acidity. Fulvic acid showed the highest total carbohydrates. The interaction between the cultivars and bio-stimulants was different in all traits. Significant differences were found among cultivars in all traits except total alkaloids, phenolics compounds, antioxidant activity in leaves, early yield, total yield, average fruit weight, TSS, and vitamin C in fruits (1st year) and number of leaves/plants, antioxidant activity, carbohydrates in leaves and total yield (2nd year).