Two field experiments were conducted at El-Qasr region in Matrouh Governorate, the North-Western Coast of Egypt, during two growing seasons of 2021/2022 and 2022/2023, to investigate the vital role of bio and organic fertilizers in facing abiotic stresses in barley fields.  The treatments include compost levels (0, 5, 10, 15 t ha^-1) as soil addition and biofertilizers i.e. nitrogen fixers strains (Azotobacter chroococcum (AZ)), phosphate dissolving bacteria (Bacillus megatherium (N)), potassium solubilizing bacteria (Bacillus circulans (R)), and their combinations relative to control (distilled water). The results showed the positive effects of biofertilizers and organic fertilizers (compost) on barley plant growth, photosynthetic activity, yield and its components. Variance analysis results (ANOVA) showed extremely significant (p < 0.05) variations among different treatments in all traits. The highest grain yields were recorded in both seasons once applied compost (15 t ha^-1) alongside a combination of (AZ+N+R) bacteria which recorded 2744.1 kg ha-1 and 873.2 kg ha-1 in the first and second seasons respectively. Also, it can be concluded that the combination of applying compost (15 t ha-1) with biofertilizer triple combination of (AZ+N+R) gave maximizing barley biomass, photosynthetic potential activity, and water use efficiency in both seasons.