The experiment was conducted in plastic pots in the laboratory of Resources Faculty, Tobruk University, Libya in 2022, using a Completely Randomized Design (CRD) in 4 replicates (each replicate contains 5 pots) to study the effect of usage fish scales as organic fertilizer on growth characteristics of white maize seedlings. Fish scales powder was added to agricultural soil pre-sowing at two different concentrations compared to the control treatment (0, 1%, and 2%). After 14 days later the results of the variance analysis showed that there were significant differences between the various concentrations. Fish scale powder adding enhanced vegetative growth characteristics such as (mean of plant height, number of leaves /seedling, mean of leaf area cm2/seedling, mean of fresh weight, as well as dry weight/seedling). Adding fish scale powder at a rate of 1% and 2% increased the mean of seedlings height by 56.67% and 114.3%, and mean of leaf area also increased by 238.9% and 476%, respectively compared to the control. Although there were no significant differences when adding fish scales powder at 1% on wet and dry weight compared to the control, adding it by 2% led to an increase in the mean of wet weight of seedlings from 8.3 to 16.06 g/seedling, also the mean of