Euphorbia milii (Christ- thorn) is a flowering succulent plant of the family Euphorbiaceae, native to Madagascar. The experiment was conducted in the flower cultivation farm, Department of Horticulture, Faculty of Agriculture, Benha University, over two consecutive years 2018/2019 and 2019/2020 to examine the effect of planting date and spraying rooted cuttings of Euphorbia milii with some growth regulators and microelements to improve vegetative growth, branching, flowering period and quality. Flower, root growth and some chemical constituents of  the Euphorbia milii plant The results showed that the highest values of vegetative and flowering growth, root parameters, and chemical compositions were obtained by using the combined treatment between planting date (autumn) and T4 100 ppm GA₃, then followed by the combined treatment between planting date (autumn) and T7 150 ppm 6-BAP in descending order. On the other hand, the lowest values of these parameters were recorded by planting date (spring) and T1 (tap water control) in both seasons. Conclusively, it was found that the combined treatment between planting date (autumn) and T4 100 ppm GA3 gave the best vegetative growth, flowering and chemical composition of Euphorbia milii.