This investigation was carried out during the 2017 and  2018 seasons, at the Experimental Farm of Horticulture Department, Faculty of Agriculture, Benha University, Egyp for studying the effect of different planting distances (20*20 and 30*30cm), and bio-fertilizers (PGRB), chemical fertilizers (NPK) and micronutrients (Zn, Fe, and Mn) on thyme plants. Results show that in each of the two cuts and the studied seasons, the maximum vegetative growth values were determined by using the combination between the planting spacing (30*30 cm), and F2 (PGRB). Hence, this combination enhanced the chemical compositions compared with most other treatments at both seasons. In addition, the essential oil percentage of thyme plant was increased by using the combined treatment of planting distances (30*30 cm) and F2. GLC analysis of thyme included 12 compounds were identified, and the main component was β-Cymene. Consequently, it is preferable to use the planting distance ( 30*30cm), and F2 (PGRB) for improving all studied parameters of thyme plant.