Two field trials were carried out during 2021 / 2022 seasons for evaluating the growth of Tuscany basil as affected by some natural foliar application (yeast and seaweed) under different aquaponic growing media. Scarcity of global resources as a result of population growth, climatic changes, and water deficit. Aquaponics (including crops and fish) have the potential to achieve high yields per unit area with limited land, water and no soil. Soilless cultivation research was carried out at the Fish Research Unit Faculty of Agriculture, Mansoura University, Mansoura city, Egypt. The growth of Ocimum basilicum 'Tuscany' grown in three different media (cocopeat + perlite mix, peatmoss + vermiculite mix and sponge) as well as the effect of spraying each of the yeast at 1, 2 and 3 g/ L and seaweed extract at1, 2 and 3 cm/L as natural materials that are environmentally friendly and improve growth, yield quality and do not affect the hydroponics system. It could be recommend to use the mixture of peat moss : vermiculite in equal proportions, as well as spraying yeast at 3 g/L every two weeks as a foliar spray for obtaining the best vegetative growth (plant height, leaf area, number of leaves/plant, fresh and dry weight), as well as the mineral contents (nitrogen, phosphorus, potassium, carbohydrates, nitrates, nitrites, content of the pigments total chlorophyll and carotenoids) of  Tuscany basil under the Egyptian conditions.