At Laboratory of Seed Testing, Faculty of Agriculture, Department of Agronomy, Mansoura University Egypt, an experiment was conducted in a lab during the month of May 2022 to improve both old and new harvested sesame crop Shandawel-3 cultivar seed germination by using some natural (without, soaking in distilled water, yeast extract at 150 and 200 ml/L, algas extract at 100 and 150 ml/L and humic acid (HA) at 200 and 300 mg/L) and chemical (without, soaking in distilled water, chitosan at 0.25 and 0.50 %, ascorbic acid (AA) at 100 and 200 mg/L and salicylic acid (SA) at 100 and 200 mg/L) substances. A unique laboratory experiment was conducted for each type of sesame seed (seed age, including both old and newly harvested seeds). Every experiment involving different seed types was conducted using a factorial experiment with a completely randomized design (CRD). Maximum values of all most studies of germination parameters were resulted from the new harvested, soaking sesame seeds in humic acid (HA) at the rate of 300 mg/L and soaking sesame seeds in ascorbic acid (AA) at the rate of 200 mg/L.  Intended for enhancing germination parameters of sesame, it could be recommended that soaking seeds in humic acid at the rate of 300 mg/L and ascorbic acid at the rate of 200 mg/L for 12 hours in  either old or new harvested seeds.