Background: The precise relationship between obesity and fertility is unknown. It seems that infertility is specifically caused by hyperandrogenism-induced long-term anovulation. Objective: The current study aimed to examine many biochemical and reproductive hormone markers in obese women. Subjects and Methods: This study included 40 women who suffer from obesity; after drawing 7–10 milliliters of blood, serum was extracted, hormones and biochemical parameters such as cholesterol, triglycerides and gonadotropins were carried out on each female. Results: The obtained results showed that both triglycerides and cholesterol levels in patient women (123.46± 9.49; 186.35 ± 5.20 mg/dL), were respectively raised (p < 0.05) compared to healthy women (2.63 ± 0.36; 5.27±0.28 mg/dL), respectively. The vitamin D values did not differ significantly (p ˂ 0.05) between the healthy (17.03± 1.91 pg/ml) and the unhealthy samples (15.26 ± 3.49 pg/ml). The results of FSH estimation in patients (6.51 ± 0.66 µIU/mL) decreased statistically (p ˂ 0.05) in comparison with healthy women (16.96 ± 5.21 µIU/mL). Likewise, the results of LH determination in patients (4.06 ± 0.35 µIU/mL) decreased statistically (p˂0.05) in comparison with healthy women (15.19 ± 5.66 µIU/mL). Conclusion: Our study's findings demonstrated that elevated levels of cholesterol and triglycerides in hyper-androgenized women who have delayed pregnancy and overweight negatively affect gonadotropins with probable infertility.