Background: Essential oils (SO) are organic substances that are extracted from plant parts, which have been thought to have anti-cancer characteristics. Aim: To investigate the potential protective effects of SO against liver toxicity induced in EAC–bearing female mice. Materials and Methods: Forty female mice were equally divided into four groups (Gps). Gp1 was served as control, Gp2 was treated with SO, Gp3 was inoculated with EAC, and Gp4 were concomitantly treated with EAC + SO. Results: Data showed that the treatment of EAC-bearing mice with SO led to an amelioration of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities. SO treatment also induced increases in the total protein levels as compared to EAC group. There was a significant improvement in the expression of GFAP protein in brain and liver tissues in the EAC/SO treated group as compared to the EAC group. Conclusion: The current results indicate to the potential hepatoprotective effects of essential oils.