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342740

Correlating DNMT1, PCNA, and RB1 genes as new prognostic biomarkers

Article

Last updated: 30 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Cellular and molecular targeting

Abstract

Hepatocellular carcinoma (HCC) can be successfully treated, and long-term survival rates can be significantly improved if diagnosed early enough. Understanding the molecular mechanisms underlying the onset and progression of HCC is critical for developing early detection methods and cutting-edge treatments. Objectives: To establish DNMT1 as a noninvasive biomarker for the diagnosis and prognosis of HCV-induced HCC, we investigated how DNA methyltransferase 1 (DNMT1), which controls DNA methylation patterns, is associated with proliferating cell nuclear antigen (PCNA) expression levels and retinoblastoma susceptibility gene (RB1) expression levels in blood samples collected from HCV-induced HCC. Design & Methods: A total of 109 HCV patients were included in this study. DNMT1 levels were measured by ELISA. PCNA and RB1 gene expression were measured using real-time PCR. Results: DNMT1 levels increased consistently and significantly in all patients as the disease progressed toward the stage of HCC. As the disease progresses, PCNA and RB1 decrease gradually. DNMT1, PCNA, and RB1 were also able to discriminate between the studied groups in terms of their diagnostic abilities. As well, the DNMT1 gene may be a prognostic or predictive factor for cirrhosis and hepatocellular carcinoma. PCNA and RB1 can also be used as prognostic and predictive markers for HCC. Conclusions: The diagnostic and prognostic results demonstrated that DNMT1, RB1, and PCNA could be useful biomarkers for detecting and predicting HCC in patients with HCV infection.

DOI

10.21608/jcbr.2024.252496.1325

Keywords

HCC, CLD, DNMT1-RB1, DNMT1-PCNA, Non-invasive biomarkers

Authors

First Name

Rady

Last Name

El-Araby

MiddleName

E

Affiliation

1. Division of Oral Biology, Tufts University School of Dental Medicine, Boston, MA 02111, USA 2. Theodor Bilharz Research Institute, Ministry of scientific Research, Cairo, Egypt

Email

radyeid.elaraby@gmail.com

City

Giza

Orcid

0000-0003-4822-5279

First Name

Marwa

Last Name

Mona

MiddleName

M.

Affiliation

Medical biochemistry Department, Faculty of Medicine ,Kafrelsheikh University

Email

marwa_mana@med.kfs.edu.eg

City

-

Orcid

-

First Name

Mariam

Last Name

Zaghloul

MiddleName

-

Affiliation

Hepatology, Gastroenterology and Infectious Diseases Department, Faculty of Medicine ,Kafrelsheikh University

Email

mariam_zaghloul@med.kfs.edu.eg

City

-

Orcid

0000-0002-4244-5396

First Name

Maha

Last Name

Morsi

MiddleName

H

Affiliation

Clinical and chemical pathology Department, Faculty of medicine, Misr University for Sciences and Technology, Giza, Egypt

Email

maha.hosni@must.edu.eg

City

-

Orcid

0000-0002-7093-0495

First Name

Mosaad

Last Name

Ibrahim

MiddleName

M

Affiliation

Medical Biochemistry, ART Unit, International Islamic Center for Population Studies and Research, Al-Azhar University, Cairo, Egypt

Email

elfaroklabm@gmail.com

City

-

Orcid

-

First Name

Wafaa

Last Name

Radwan

MiddleName

M.

Affiliation

Clinical pathology Department, Faculty of medicine. Menoufia University

Email

wafaa3rad@yahoo.com

City

-

Orcid

-

First Name

Amr

Last Name

Rezk

MiddleName

A

Affiliation

Clinical pathology Department, Faculty of medicine, Al-Alazhar University, Cairo, Egypt.

Email

dramrelgaidy@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Shaheen

MiddleName

A

Affiliation

Clinical pathology Department, Faculty of medicine, Al-Alazhar University, Cairo, Egypt.

Email

dr.mashaheen85@gmail.com

City

-

Orcid

-

First Name

Walid

Last Name

Abusheir

MiddleName

A

Affiliation

-Biology department, faculty of Science and Arts, Albaha University, KSA -Zoology Department, faculty of science, Alazhar University, Egypt

Email

walid_rock1@yahoo.com

City

-

Orcid

-

First Name

Yasmine

Last Name

Elhusseny

MiddleName

-

Affiliation

Lecturer of Biochemistry and Molecular Biology, School of Medicine, Newgiza University, Giza,Egypt

Email

yasmine.youns@ngu.edu.eg

City

-

Orcid

-

First Name

Reham

Last Name

Othman

MiddleName

F

Affiliation

Lecturer of medicine, School of Medicine, Newgiza University, Giza,Egypt.

Email

reham.fekry@ngu.edu.eg

City

-

Orcid

-

First Name

Hany X

Last Name

Elghobary

MiddleName

A

Affiliation

Clinical and chemical pathology Department, Faculty of medicine, Cairo University, Egypt.

Email

hany.alghobary@gmail.com

City

-

Orcid

-

Volume

8

Article Issue

1

Related Issue

46253

Issue Date

2024-03-01

Receive Date

2023-12-01

Publish Date

2024-03-01

Page Start

17

Page End

28

Print ISSN

3009-6391

Online ISSN

3009-7312

Article File

JCBR_Volume 8_Issue 1_Pages 17-28.pdf

PDF . 1.6MB

Link

https://jcbr.journals.ekb.eg/article_342740.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=342740

Order

2

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

Egyptian Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

Correlating DNMT1, PCNA, and RB1 genes as new prognostic biomarkers

Details

Type

Article

Created At

30 Dec 2024