The study was conducted in a pomegranate field orchard at the Agricultural Research and Experiments Station of the College of Agriculture, University of Kirkuk, Iraq, located in the Al-Sayadah area, during the 2023 growing season, on young pomegranate trees of var. Wonderful. The experiment included two factors: the first factor included five levels of organic fertilizers (0, 250, and 500 g/tree of poultry waste and two levels of 15 and 30 g/tree of humic acid) and three amino acid concentrations (0, 5, and 10 ml/L). Results showed that applying 500 g/tree of poultry waste significantly increased the mineral content of pomegranate trees (carbohydrate percentage in the branches, nitrogen in the branches, and the carbohydrate/nitrogen ratio in the branches) at rates of 4.667%, 1.721%, and 2.771%, respectively. There was also a significant increase in potassium content in the leaves at a rate of 0.655% with the application of 30 g/tree of humic acid. A concentration of 5 ml/L of amino acids enhanced the leaf protein percentage by 6.762%, while 10 ml/L was most effective for the majority of the studied characteristics (potassium in the leaves, carbohydrates in the branches, nitrogen percentage, and the carbohydrate/nitrogen ratio in the branches) at rates of 0.692%, 3.814%, 1.387%, and 2.760%, respectively, compared to unsprayed trees. As for the binary interaction between the two factors, it showed a significant effect on all the traits studied.