The study aimed to evaluate the biocontrol efficacy of native Streptomyces spp. isolates against Pectobacterium carotovorum subsp. carotovorum (Pcc), the causal agent of potato soft rot. Nine Pectobacterium carotovorum subsp. carotovorum isolates were obtained from potato tubers exhibiting typical symptoms of soft rot and were identified based on morphological and biochemical characteristics. The pathogenicity of these isolates was assessed, and the most virulent isolate, Pcc6, was identified through PCR amplification and sequencing of the 16S rRNA gene. Additionally, sixteen actinomycete isolates were identified as Streptomyces spp. based on thorough assessment of their physiological and biochemical characteristics. In vitro antagonism tests revealed significant variation in the inhibitory effect of Streptomyces spp. isolates on the growth of Pcc6, with isolates 1, 3, 4, 5, 6, 7, 8, 9, 10, and 12 demonstrating strong inhibitory activity and were selected for further experiments. Furthermore, in vivo tuber inoculation tests and pots experiments demonstrated the biocontrol efficacy of selected Streptomyces spp. isolates against Pcc6 in potato tubers, with isolate 4 exhibiting the highest bioagent efficiency, completely preventing the soft rot lesion in vivo tuber inoculation test and reducing the disease severity by 93.94% in the pots experiment. The findings of this study underscore the promising role of Streptomyces spp. isolates as biocontrol agents against Pectobacterium carotovorum subsp. carotovorum, providing valuable insights for the development of sustainable disease management strategies in potato production.