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286228

Identification of faba bean (<i>Vicia faba</i> L.) protein by electrophoresis

Article

Last updated: 04 Jan 2025

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Abstract

The seeds of fifteen faba bean varieties and genotypes were used in this study; Giza 716, Giza 843, Noubariaa 3, Wady 1, Sakha 1, T. W, hybrid 21, hybrid 22, hybrid 23, hybrid 24, Sakha 4, Noubariaa 1, Giza 40, Missr 1 and Giza 3. The chemical composition of all the previously mentioned varieties and genotypes was done. Results indicated that, carbohydrate content ranged from 51.11-58.15%, protein content ranged from 24.60%-30.68%, moisture from 9.07-10.57%, fat content from 1.54- 2.70% and ash content from 1.38 - 4.16%. Proteins were fractionated by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS–PAG). Results revealed that Giza 3, Giza 40, Sakha 4, Noubariaa 1 and T.W were resolved into 8 bands, Giza 716, Giza 843, hybrid 22 and Missr1 were resolved into 9 bands, while hybrid 24, hybrid 23, Wady 1, and Sakha 1 were resolved into 10 bands. Also, hybrid 21 and Noubariaa 3 were resolved into 11 bands. The molecular weight of protein subunits ranged between 15.00 and 352.50 kDa.

DOI

10.21608/aasj.2022.286228

Keywords

Faba bean, Gel Electrophoresis, SDS–PAG, molecular weight, dodecyl sulfate polyacrylamide

Authors

First Name

A.

Last Name

Yassen

MiddleName

A. M.

Affiliation

Department of Agronomy-Biochemistry, Faculty of Agriculture, Al-Azhar University, Assiut Branch, Egypt

Email

ahmedyassin.el.8.61@azhar.edu.eg

City

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Orcid

-

First Name

M.

Last Name

Taha

MiddleName

G. A.

Affiliation

Department of Agriculture-Biochemistry, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Ibrahim

MiddleName

M.

Affiliation

Department of Agronomy-Biochemistry, Faculty of Agriculture, Al-Azhar University, Assiut Branch, Egypt

Email

-

City

-

Orcid

-

First Name

Y.

Last Name

Khalifa

MiddleName

A. M.

Affiliation

Department of Agronomy-Biochemistry, Faculty of Agriculture, Al-Azhar University, Assiut Branch, Egypt

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-

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-

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Volume

5

Article Issue

3

Related Issue

42543

Issue Date

2022-12-01

Receive Date

2022-09-21

Publish Date

2022-12-01

Page Start

261

Page End

274

Print ISSN

2535-1680

Online ISSN

2535-1699

Link

https://aasj.journals.ekb.eg/article_286228.html

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https://aasj.journals.ekb.eg/service?article_code=286228

Order

38

Type

Research article

Type Code

764

Publication Type

Journal

Publication Title

Archives of Agriculture Sciences Journal

Publication Link

https://aasj.journals.ekb.eg/

MainTitle

Identification of faba bean (<i>Vicia faba</i> L.) protein by electrophoresis

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Article

Created At

30 Dec 2024