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294994

Utilization of Spectrophotometric Technique for the Quantitative Evaluation of Cefadroxil Monohydrate in its Pure form and Its Therapeutic Doses

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Last updated: 03 Jan 2025

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Abstract

Establishing and adopting a quick, easy, and cheap method for determining antibiotic Cefadroxil monohydrate (CFL) in its pure form and pharmaceutical preparations. The method involves adopting spectrophotometry in the visible region. The method adopted the oxidation of the studied medicine by an excess amount of N-Bromo succinamide (NBS), then estimated the NBS Excess by bleaching the dye color of Methylene Blue. The maximum wavelength was measured at λ 586 nm. This approach obeys the Beer-Lambert law for the range (5-20 µg. ml-1), with a correlation coefficient of 0.9980. The calculated molar absorptivity was 1.41× 103 (L. mol-1. cm-1), and Sandall's sensitivity was 0.27 µg. cm-2. The limit of detection (LOD) was 3.508 µg. ml-1, as well as the limit of quantification (LOQ), was 11.693 µg. ml-1. The precision and accuracy of the method are established and checked.
            This study measured the optimal reaction conditions and other analytical variables. It showed decent repeatability, and the relative standard deviation (RSD percentage) was less than 1%.
           The proposed methodology identified the effective implementation of an easy, resilient as well as precise spectrophotometric method to determine Cefadroxil employing N-Bromo succinimide as Cefadroxil oxidant factor and also the unreacted N-Bromo succinimide bleached the methylene blue dye. The process has been successfully extended in different pharmaceutical formulations to evaluate the Cefadroxil drug.

DOI

10.21608/eajbse.2023.294994

Keywords

Spectrophotometric, indirect oxidation, Cefadroxil monohydrate, antibiotic, pharmaceutical forms

Authors

First Name

Basim

Last Name

Aldhalemy

MiddleName

F.

Affiliation

Department of Chemistry, Faculty of Sciences, University of Kufa, Najaf 54001, Iraq,

Email

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City

Iraq

Orcid

-

First Name

Mohauman

Last Name

Al-Rufaie

MiddleName

M. M.

Affiliation

Department of Chemistry, Faculty of Sciences, University of Kufa, Najaf 54001, Iraq,

Email

muhaimin.alrufaie@uokufa.edu.iq

City

najaf

Orcid

-

Volume

15

Article Issue

1

Related Issue

39738

Issue Date

2023-06-01

Receive Date

2023-03-11

Publish Date

2023-04-16

Page Start

61

Page End

72

Print ISSN

2090-0783

Online ISSN

2090-0856

Link

https://eajbse.journals.ekb.eg/article_294994.html

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https://eajbse.journals.ekb.eg/service?article_code=294994

Order

294,994

Type

Original Article

Type Code

687

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, E. Medical Entomology & Parasitology

Publication Link

https://eajbse.journals.ekb.eg/

MainTitle

Utilization of Spectrophotometric Technique for the Quantitative Evaluation of Cefadroxil Monohydrate in its Pure form and Its Therapeutic Doses

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Type

Article

Created At

30 Dec 2024