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341442

Prevalence, Antimicrobial Susceptibility, and Virulence Gene Profile of Enterococcus Species isolated from Some Farmed Fish Retailed in Zagazig City, Egypt

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

Animal Health, Nutrition and Food Control (Veterinary Public Health, Animal Wealth Development, Animal Nutrition, Zoonoses, Food Control)

Abstract

The current study aimed to assess the prevalence of Enterococcus species isolated from some farmed fish species, including tilapia (Oreochromis niloticus), brush tooth lizard (Saurida undosquamis), horse mackerel (Trachurus trachurus), and red porgy (Pagrus pagrus) sold in Zagazig city, Sharkia Governorate, Egypt. Additionally, antimicrobial susceptibility testing was performed for Enterococcus species using the disc diffusion method. Furthermore, multiplex PCR was performed to identify virulence-associated genes of Enterococcus faecalis isolates. The results indicated contamination of the examined fish with various Enterococcus species with an overall total prevalence of 52.5%. The identified Enterococcus species were E. faecalis (26.25%), E. faecium (15%), and E. hirae, E. raffinosus, and E. durans (3.75%, each). The antibiotic sensitivity test revealed variable resistance patterns of the retrieved isolates to various antimicrobial agents, such as kanamycin (100%), clindamycin (76.9%), sulfamethoxazole (69.2%), ampicillin (69.2%), and colistin (61.5%). PCR screening of virulence genes revealed that E. faecalis harbored sodA (100%), gelE (83.3%), and ace (50%) genes. Consequently, urgent measures are needed to implement hygienic practices to control microbial contamination in both the aquatic environment and fish markets.

DOI

10.21608/zvjz.2024.262051.1229

Keywords

fish, E. faecalis, E. faecium, Antimicrobial susceptibility, Virulence genes

Authors

First Name

Mohamed

Last Name

Mohamed Hussein

MiddleName

Abdallah

Affiliation

Food Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

Email

elged2010@gmail.com

City

Zagazig

Orcid

-

First Name

Ahmed

Last Name

El-tahlawy

MiddleName

Sabry

Affiliation

Food Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

Email

aseltahlawy@vet.zu.edu.eg

City

Zagazig

Orcid

0000-0002-4506-0168

First Name

Hend

Last Name

Abdelmoneim

MiddleName

Mohamed

Affiliation

Food Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

Email

hanoda19995@gmail.com

City

Zagazig

Orcid

-

First Name

Rasha

Last Name

El Bayomi

MiddleName

Mohamed

Affiliation

Food Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

Email

rmazab@zu.edu.eg

City

Zagazig

Orcid

-

First Name

Karima

Last Name

Abdallah

MiddleName

Mohamed Eissa

Affiliation

Food Hygiene, Safety, and Technology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt

Email

karimaeissa1989@gmail.com

City

Zagazig

Orcid

-

Volume

52

Article Issue

1

Related Issue

47003

Issue Date

2024-03-01

Receive Date

2024-01-10

Publish Date

2024-03-30

Page Start

35

Page End

48

Print ISSN

1110-1458

Online ISSN

2357-075X

Link

https://zvjz.journals.ekb.eg/article_341442.html

Detail API

https://zvjz.journals.ekb.eg/service?article_code=341442

Order

341,442

Type

Original Article

Type Code

601

Publication Type

Journal

Publication Title

Zagazig Veterinary Journal

Publication Link

https://zvjz.journals.ekb.eg/

MainTitle

Prevalence, Antimicrobial Susceptibility, and Virulence Gene Profile of Enterococcus Species isolated from Some Farmed Fish Retailed in Zagazig City, Egypt

Details

Type

Article

Created At

30 Dec 2024