This study was conducted to evaluate the effect of the partial and total substitution of live algae in a green water system with stain in seabream (Sparus aurata) larval rearing, three treatments were applied green water treatment (algae treatment), 50% algae and 50% stain treatment (mixed treatment) and 100% stain treatment (stain treatment). Water quality, body length, survival rate, percentage of deformity were measured, and the results revealed that higher dissolved oxygen were maintained at mixed treatment (5.14ppm), no significant differences in total length between all treatments, the final body weight at day 120 of big size group was higher in mixed treatments (0.26 gram) in the end of experiment.  mixed treatment had the highest number of big size group (15%) and the lowest number of small size group (43%), survival rate at day 60 was highest in the algae treatment (22.77 %) followed by mixed treatment (22.76 %) with no significant differences, survival rate from day 60 till day 120 after hatch was higher in mixed treatment (50.20%) than algae treatment (49.6%), the incidence of deformities was low at all treatments. It could be concluded that the stain could substitute live algae with good results.