The purpose of this study was to examine the impact of spraying putrescine (at concentrations of 10, 20, and 30 ppm) and/or chitosan (at concentrations of 0.1%, 0.2%, and 0.3%) three times per year on the yield, physical properties, and chemical properties of Manfalouty pomegranate trees that are grown in sandy loam soil. Spraying putrescine and chitosan on pomegranate trees leads to improved fruit production and better fruit physicochemical properties. This positive effect is gradual and parallel to the increase in the concentration of each material used. When both materials were used in combination, regardless of the concentration, they were more effective in enhancing yield and improving fruit quality as compared to trees treated with either material alone or untreated trees. Moreover, trees that received the highest concentration of both materials in combination produced the highest yield and best quality fruit during the two experimental seasons. Additionally, there were no significant differences between the two highest concentrations during the two seasons of the experiment.