Fungi are considered one of the most important sources relied upon in the production of biodiesel, especially if the materials to be used are agricultural waste and are considered an inexpensive source of sugars. Aspergillus terreus Mekky221 was the most abundant of the Seven oleaginous fungal isolates that were isolated from Agriculture waste specimens were taken from cultivated land. and subsequently examined for biodiesel production in the current study. By using morphological (macroscopic and microscopic) analysis and molecular confirmation using 18S rRNA sequencing, the isolate's species identity was established and recorded on Gene Bank under accession number PQ182607. In order to enhance lipid accumulation, we also improved the culture conditions, Aspergillus terreus Mekky221 showed the highest lipid production (Dray biomass 4.89 ± 0.19 g/L giving lipid content 2.52 ± 0.06 g/L Equivalently 51.57 ± 3.03 %) in the existence of 100g/L sucrose and 10 g/L yeast extract at pH, 5 after five days of incubation at 30°C under static conditions. solid state fermentation medium was made: A 5-liter flask was filled with 40g of dry bagasse and rice straw as inexpensive carbon sources, mixture evenly moistened at a 60% level using sterile distilled water and peptone (5 g/1), autoclaved after cooling, spores added with known concentration, pH 5, temp. 30 and incubated for 5 days Aspergillus terreus Mekky221 showed lipid production (dray biomass 3.99 ± 0.17 g/L giving lipid content 2 .11 ± 0.08 g/L Equivalently 52.8 ± 0.13 %). Finally, the transesterification process is dependent on the one-step conversion of the fungal dry biomass's lipid to FAME and by using a gas chromatographic analysis of fatty acids. Aspergillus terreus Mekky221 is therefore thought to be a promising oleaginous filamentous fungus that can be used to biodiesel production from agricultural waste.