species causing diabetic foot. Diabetic foot ulcers are complex infections arising from a combination of microbial and host factors. DFUs typically harbor a diverse array of microorganisms, encompassing aerobic and anaerobic bacteria, fungi, and occasionally viruses. Frequent bacterial culprits in diabetic foot ulcers are Staphylococcus spp, and a variety of gram-negative rods. In particular swabs were taken from diabetic individuals and cultivated in conditions that were conducive to the growth of bacteria. Following that, they underwent diagnosis using the VITEK2 system in the laboratory. The VITEK 2 System is an automated microbiology system used primarily for the identification and antibiotic susceptibility testing of bacteria 16SrRNA gene (plays a crucial role in the process of translating genetic information from mRNA into proteins). The gene encoding 16SrRNA is commonly used in molecular biology for various applications, particularly in the identification and classification of microorganisms), isolates saved for the additional examination of gene sequencing to determine the type of bacteria. A total of 50 swabs were positive for bacterial culture, the percentage of Staphylococcus spp, was 30% compared to Enterobacteriaceae spp, was 12% while other bacterial causes were 58% of the total percentage100% included (Achromobacter, Wautersiella falsenii, Proteus alimentorum). The current article concludes with the identification of bacterial species in diabetic foot ulcers, phenotypic and laboratory diagnosis, and selecting pure isolates for diagnosis confirmed by 16S rRNA gene sequencing.