This study was conducted in the period from July 27 to October 2023, when 60 samples were collected, taking (30 samples from the cities of Abache and Oum-Hajar) in Chad.  It is 15 representative samples weighing 2.5-3 Kg. The samples were packed in sterile plastic bags and transferred to the bacteriological laboratory at the Center for Food Quality Control (CECOQDA) for analysis.  The selection of production areas is based on the abundance of lime flour at all times in those areas. This study included bacterial species (fecal E. coli, Salmonella, Staphylococcus, FAMT, Bacillus cereus, Lactic acid bacteria, and Coliformes).  The microbial load of total aerobic bacteria in the lime samples for the cities of Abache and Oum-Hajar ranged respectively (1.10⁵ - 9.77.10² and 1.00.10 - 6.73.10²), and the bacterial load of coliforms in the lime samples for the cities of Abache and Oum-Hajar ranged (1.10 - 7.77.10⁴ and 1.10-00) While checking for salmonella, the result was negative.  As for the investigation of the microbial load of Staph. aureus bacteria in the cities of Abache and Oum-Hajar, respectively, it ranged between (1.10² - 8.00.10 and 1.10² - 5.31.10⁴).  Statistical analysis showed a very strong relationship between total aerobic bacteria, Staphylococcus aureus, and lactic acid bacteria. While the investigation of E- coli and Bacillus cereus bacteria falls within the standards of the Codex Alimentarius (7052/2005/FAO). Therefore, consuming lime does not pose a risk to human health. The microbial load obtained in our studied samples is associated with various sources of contamination such as poor sanitary practices at the level of producers and at the level of market sellers, the use of wastewater, poultry waste, soil contamination, cross-contamination, etc. Statistical analyzes were performed using. ANOVA was conducted using the recommended R software version (Rx64 3.2.5) for Windows16.  The confidence level for these results was set at P≤0.05.