Abstract

Skeletal muscle-adipose tissue crosstalk is crucial for developing therapeutic strategies for various metabolic disorders. While obesity is known to disrupt microRNA (miRNA) expression profiles in skeletal muscle, a comprehensive understanding of this phenomenon remains elusive. Therefore, our study aims to investigate the impact of high-fat diet (HFD)-induced obesity on miRNA dysregulation within skeletal muscle tissue at distinct time points. In HFD model, rats received HFD for 2, 4, 6, 8, or 10 weeks. Body weight was determined at 2, 4, 6, 8, and 10 weeks. At the end of each interval, group of animals (n = 8) were sacrificed and skeletal muscle tissues were harvested to assess miRNAs expression levels. HFD administration for 8 and 10 weeks resulted in marked weight changes in comparison to control group. There were not much significant changes in body weight seen in low durations of HFD feeding. Alterations in miR130a, miR30a-5p, miR133a-5p, miR193a-5p, and miR125a-5p expression levels were observed at different time point relative to control rats. While miR let-7 and miR107-5p were upregulated at all-time points compared to control animals. Thus, skeletal muscle miRNA dysregulation likely plays a role in HFD-induced obesity.