Sugarcane by-products have the efficacy of alternative, low-cost culture media that could be used for the cultivation of a wide range of fungi. Various formulations of culture media are used for the growth of fusarium species which can provide a variety of famous bioactive compounds. This study investigates the bioactive compounds and biological activities of the fungal strain Fusarium solani when cultivated on sugarcane by-products as a different carbon sources (bagasse, vinasse, and molasses). The obtained results revealed that the highest growth rate of Fusarium solani detected in T9 (molass: vinasse with a ratio 1:1). The maximum phenols and flavonoid content was recorded by fungal strain grown on the same treatment (T9) with a concentration of 360.09 and 111.56 mg/mL, respectively. Fusarium solani exhibited the highest free radical scavenger in T9 and T11 (molass: vinasse with a ratio 1:3). According to the previous F. solani cultivated on T9 produced a high concentration of active metabolites (48.02 mg/L) which was analyzed by HPLC analysis. In addition to that, the fungus showed better antibacterial activity against Staphylococcus aureus when grown on T9. This finding suggests that sugarcane industrial wastes can be used as a growth culture economic medium for Fusarium solani as this fungus has a various useful pharmaceutical and agricultural applications due to its produced secondary metabolites that exhibit potential antioxidant and antibacterial activities.