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339161

Enhanced Anticancer Efficacy of Noscapine-Loaded Lipid Nanocapsules: In-vitro and In-vivo Evaluation.

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Biochemistry

Abstract

Noscapine (NOS), an alkaloid with several interesting biological applications, has demonstrated antitumor action and cough suppressant potential. However, the strong hydrophobic nature of NOS makes it poorly water-soluble. Therefore, it was necessary to figure out how to make NOS more soluble. In this study, we investigated the anticancer effect of free NOS as compared to that of NOS-loaded lipid nanocapsules (NOS-LNCs) on the 4T1 cell line, and in an orthotopic breast cancer (BC) mouse model. Spherical-shaped NOS-LNCs were prepared with a particle size (PS) of 213.4 ± 1.41 nm and a zeta potential (ZP) of -13.6 ± 1.17. The IC50 of free NOS, NOS-LNCs, and plain LNCs was measured on the 4T1 cell line by MTT assay, and the results revealed that the IC50 was 66.107 µg/ml, 13.209 µg/ml, and 121.132 µg/ml, respectively. On the other hand, NOS-LNCs demonstrated stronger anticancer effects in-vivo along with strong antiproliferative and antioxidant properties. Also, the immunohistochemistry analysis of BAX and BCL-2 in the tumor tissue confirmed the apoptotic activity of the NOS-LNCs, as it increased the expression of BAX and downregulated BCL-2. In conclusion, the current investigation showed that the developed NOS nanoform has a great deal of potential as a therapeutic agent for the treatment of BC.

DOI

10.21608/ejchem.2024.259976.9129

Keywords

Noscapine, breast cancer, anticancer effect, Lipid nanocapsules

Authors

First Name

Nadeen

Last Name

Abdel-Wahab

MiddleName

Diaa

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

nadeen.diaa@mans.edu.eg

City

Mansoura

Orcid

0000-0002-0338-5730

First Name

Mohamed

Last Name

Kabil

MiddleName

Fawzi

Affiliation

Nanomedicine Research Labs, Center for Materials Science, Zewail City of Science and Technology, 6th of October City, Giza, Egypt

Email

p-mkabil@zewailcity.edu.eg

City

Giza

Orcid

-

First Name

Ibrahim

Last Name

El-sherbiny

MiddleName

M.

Affiliation

Nanomedicine Research Labs, Center for Materials Science, Zewail City of Science and Technology, 6th of October City, Giza, Egypt

Email

ielsherbiny@zewailcity.edu.eg

City

Giza

Orcid

-

First Name

Mohamed

Last Name

Salama

MiddleName

F.

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

mfouda@mans.edu.eg

City

Mansoura

Orcid

-

First Name

Gehad

Last Name

El-Sayed

MiddleName

-

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

elsayedgehad89@gmail.com

City

Mansoura

Orcid

-

First Name

El-Said

Last Name

El-Sherbini

MiddleName

-

Affiliation

Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura University, Egypt

Email

sshrbini@mans.edu.eg

City

mansoura

Orcid

-

Volume

67

Article Issue

8

Related Issue

47534

Issue Date

2024-08-01

Receive Date

2024-01-01

Publish Date

2024-08-01

Page Start

409

Page End

422

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 67_Issue 8_Pages 409-422.pdf

PDF . 912.9kB

Link

https://ejchem.journals.ekb.eg/article_339161.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=339161

Order

339,161

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Enhanced Anticancer Efficacy of Noscapine-Loaded Lipid Nanocapsules: In-vitro and In-vivo Evaluation.

Details

Type

Article

Created At

30 Dec 2024