Sarcophyton Convolutum extract (S) is rich source of bioactive secondary metabolite. In this article we report identification of major components and study antimicrobial, anticancer and antiviral activities of non-polar Sarcophyton Convolutum extract. Results demonstrated that S. Convolutum extract (S) has strong bactericidal effect against P. aeruginosa ATCC 15442 recording MIC value about 0.05µg/mL. S. Convolutum extract (S) showed cytotoxicity against hepatocellular carcinoma cell line (HepG2), human colorectal adenocarcinoma (CaCo2), breast cancer cell line (MCF-7), and pulmonary adenocarcinoma (A549) and recorded IC50 of 102.73, 100.62, 86.68 and 81.64 while antiviral activity towards HSV-1 and HAV virus at the maximum nontoxic concentration was 32.69% and 11.53%, respectively. GCMS analysis of S. Convolutum extract (S) showed about 25 compounds about 45.31% of extract is sesquiterpene, 47.53 % fatty acid derivatives 3.13% steroids and 2.2% is retinal diterpene. Our in vitro study indicates that sesquiterpene rich softcoral S. Convolutum extract (S) is a promising anticancer and antimicrobial agent but weak antiviral agent.