Nowadays, there is a growing concern regarding the conservation of ecological systems. The continuous usage of polymers greatly contributes to environmental pollution. Consequently, the development to eliminate environmental pollution. Therefore, the use of locally sourced biomaterials in polymer composites promotes environmental awareness, reduces reliance on unsustainable synthetic materials, and has the advantage of lower cost of natural fibers compared to synthetic alternatives. The key objective of this research is to establish an environmentally friendly nanocomposite (NCs) film by utilizing natural resources. To achieve this, the polylactic acid-cellulose acetate-curcumin-silver (PLA-CA-CUR-Ag) NCs film was synthesized in four steps using a simple solution-casting and ultrasonic irradiation. Acetylation of cellulose (CE) extracted from sukkari palm leaf using acetic anhydride, AcOH and H2SO4 catalyst. Additionally, CUR was transformed into nanoparticles (NPs) using physico-chemical fabrication techniques. Ag NPs were synthesized using the solid-state approach, utilizing the pomegranate peel. These NPs and CA were distributed in PLA as the supporting matrix. The structure of the novel synthesized NCs film was proved by several tools as FT-IR, XRD, UV, SEM, TGA, and DTG. In addition to revealing the importance of synthesized NCs film, as they have proven antibacterial and antifungal effectiveness. CA, CUR NPs, and Ag NPs increased the activity of PLA against Bacillus subtilis and Candida albicans, whereas PLA alone did not show any activity.