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Rapid RP-HPLC Detection Method For Quantification Of Gentamicin Sulfate Loaded Wound Dressing Nanofiber Formulation With Accelerated In Vivo Wound Healing

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Biochemistry

Abstract

Wound healing is a complex and dynamic procedure which demands a proper climate to reinforce quick remedy. Gentamicin is a broad spectrum amino glycoside antibiotic having a potent bactericidal impact, but its implication is restricted by the minimal bioavailability, shortened half-life, and nephrotoxicity. In this work, a controlled release gentamicin sulfate formulation was constructed employing PGA-HA/PVA wound dressing nanofiber. The loss of UV chromophores in gentamicin structure restrains a straightforward emergence of analytical method. In this context, a validated reversed-phase high-performance liquid chromatography method for the rapid quantification of gentamicin sulfate content in the nanofiber delivery system was developed. The nanofibrous scaffold were constructed from hyaluronic acid, polyvinyl alcohol, and Polygalacturonic acid for accelerating wound healing. The morphology of nanofibers were checked by SEM. The mean diameter of the nanofibers were 360 nm. The FT-IR characterizations disclosed the incorporation of gentamicin into the nanofibers. The presence of gentamicin induces changes in the mechanical properties via influencing the material's ability to withstand tensile forces and deformation. In vivo wound healing on the wistar rats disclosed a faster healing for animals treated with PGA-GM-HA/PVA nanofiber that confirmed by histopathological examination compared to those applied sterile gauze.

DOI

10.21608/ejchem.2024.252585.8939

Keywords

RP-HPLC, Wound dressing nanofiber, gentamicin, Sterile gauze, Electrospinning, Polygalacturonic acid, and Hyaluronic acid

Authors

First Name

Mona M.

Last Name

Agwa

MiddleName

-

Affiliation

Department of Chemistry of Natural and Microbial Products, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.

Email

magwa79@gmail.com

City

Alexandria

Orcid

0000-0003-0232-7616

First Name

Mohamed R.

Last Name

El-Aassar

MiddleName

-

Affiliation

Department of Chemistry, College of Science, Jouf University, Sakaka 2014, Saudi Arabia. , Polymer Materials Research Department, Advanced Technology and New Materials Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt.

Email

mohamed.elaassar@gmail.com

City

Sakaka- 2014, Saudi Arabia

Orcid

-

First Name

Rehab I.

Last Name

Moustafa

MiddleName

-

Affiliation

Department of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt.

Email

rehabib_m@yahoo.com

City

-

Orcid

-

First Name

Hassan

Last Name

Elsayed

MiddleName

-

Affiliation

Department of Microbial Biotechnology, Biotechnology Research Institute, National Research Centre, Dokki, Giza 12622, Egypt.

Email

hassanaboelnor@yahoo.com

City

-

Orcid

-

First Name

Nagham G.

Last Name

El-Beheri

MiddleName

-

Affiliation

Polymer Materials Research Department, Advanced Technology and New Materials Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, Universities and Research Institutes District, Alexandria 21934, Egypt.

Email

nagham.elbeheri.1512@gmail.com

City

-

Orcid

-

Volume

67

Article Issue

6

Related Issue

47152

Issue Date

2024-06-01

Receive Date

2023-12-02

Publish Date

2024-06-01

Page Start

445

Page End

456

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 67_Issue 6_Pages 445-456.pdf

PDF . 1.3MB

Link

https://ejchem.journals.ekb.eg/article_337645.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=337645

Order

337,645

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Rapid RP-HPLC Detection Method For Quantification Of Gentamicin Sulfate Loaded Wound Dressing Nanofiber Formulation With Accelerated In Vivo Wound Healing

Details

Type

Article

Created At

30 Dec 2024