The construction and development of polyvinyl chloride (PVC) membrane sensors for the determination of moxifloxacin (MOX) were studied. Three different membrane sensors were created by incorporating 4-tert-butylcalix[8]arene (sensor 1), β-cyclodextrin ( sensor 2)and γ-cyclodextrin (sensor 3) as ionophores. In a PVC matrix, o-nitrophenyl octyl ether (o-NPOE) was used as a plasticizer and potassium tetrakis (4-chlorophenyl) borate (KTPClPB) as an ion additive. The construction of reaction mechanisms has been facilitated by the formation of supramolecular inclusion complexes between drug and ionophores. The calixarene, β-CD, and γ-CD sensors exhibited a response to moxifloxacin that closely followed the Nernstian behavior within the pH range of 3 to 8. The proposed sensors exhibited a calibration range for MOX from 1x10-2 - 4.3x10-6, 1x10-2 - 3.3x10-6 and 1x10-2 - 3.4x10-6, with slope 54, 55 and 56 mV decade−1 and the detection limits were 1.3 ×10-6, 1×10-6, and 1.03×10-6 for sensors 1, 2, and 3, respectively. The interference study of the investigated method was examined, and the low values of the selectivity coefficient indicate that the sensors showed high selectivity for MOX. The developed sensors exhibited favorable relative standard deviation and high recovery for MOX. The sensors were successively used for the evaluation of MOX in a pharmaceutical formulation and spiking urine samples. The results obtained by the sensors are strongly compatible with those of the reported methods. On the other hand, the advanced sensors are used as indicator electrodes for the titration of MOX with NaTPB potentiometrically.