Carotenoids of natural origin are classified as secondary metabolites known biological activities such as anti-inflammatory, anti-cancer, and antioxidant effects. The exploration of novel natural sources of carotenoids has been driven by the demand for these compounds in several industries, including food, textiles, cosmetics, and pharmaceutical. This research is intended to study the isolation and identification of strains producing orange carotenoid pigment (OCP), the extraction of the pigment from the isolated strain, the chemical characterization of the pigment and testing its antioxidant, antimicrobial and antibiofilm properties. By using morphological traits and 16S rRNA sequencing, the isolate was recognized as Planomicrobium sp. GMMA, with accession number OM921394. The OCP from Planomicrobium sp. was first confirmed for purity and characterization by UV, FTIR and LC-ESI-MS/MS spectrophotometer. In the pigment extract of Planomicrobium sp., nine compounds were tentatively identified, including eight carotenoid derivatives and one chlorophyll. The obtained LC-MS spectra showed molecular ion peaks at m/z [M+H] 536.05, 224.1, 553.22, 570.1, 499.5, 600.16, 644.2 and 706.2, which lead to a molecular weight of 536, 224, 552, 568.9, 497.7, 600, 642.9, and 704 g/mol, which refers to Lycopene, Kocumarin, β-Cryptoxanthin, zeaxanthin, Neurosporaxanthin, Violaxanthin, Dinoxanthin and Flavuxanthin, respectively. The molecular ion peaks at m/z [M-H] 895.2, which leads to a molecular weight of 895.47 g/mol, which refers to chlorophyll. The OCP had biological activity considerable radical scavenging activity with DPPH, ABTS, ferrous ions (Fe2+), and nitric oxide. Also, OCP showed good antimicrobial activity against the microorganism's test. The MIC and MBC values of OCP against Gram-positive and Gram-negative bacteria ranged from 10 to 40 µg/ml. Therefore, the antibiofilm activity of the OCP was moderate; it showed 58.41 and 54.73% for S. aureus and E. coli, respectively.