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Gallic Acid-Loaded Microemulsion Combined with Low Doses of Radiation Ameliorates Chronic Pancreatitis in Rats via Modulation of the Nrf2-Keap1-HO-1 Pathway

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Biochemistry

Abstract

Chronic pancreatitis (CP) is a complicated inflammatory disease with a significantly reduced quality of life and irreversible pancreatic damage. Gallic acid (GA), a common secondary polyphenol metabolite generated from plants, stimulated nuclear translocation of Nrf2 as well as the production of target proteins such as heme oxygenase-1 (HO-1). However, its usage is restricted due to its low water solubility, which results in poor bioavailability. So, in the current study, the microemulsions (MEs) were prepared based on polyvinylpyrrolidone polymer (PVP) as a carrier for GA to increase its miscibility and bioavailability. Also, characterization of polymer ME system was evaluated by using FTIR and DLS, in addition to studying its antimicrobial activity toward some types of bacteria. The objective of this research was to evaluate the effect of PVP/GA microemulsion prepared with clove oil essential oil (CEO) combined with low doses of radiation to ameliorate chronic pancreatitis in rats via modulation of the Nrf2/Keap1/HO-1 Pathway. 42 rats were randomly assigned to seven groups: control group, rats were received PVP/CEO/GA microemulsion (0.9 ml/100 g body weight) orally 5 days per week for 4 weeks, rats were exposed to low doses of radiation (0.25 Gy every week for 4 weeks), rats were received L-arginine 4 times by a dose of 250 mg/100 g body weight (2 doses separated by 1-hour interval), every week for 4 weeks to induce chronic pancreatitis, rats were received L-arginine & were received GA-loaded PVP/CEO microemulsion, rats were received L-arginine & were exposed to low doses of radiation, and rats were received L-arginine & were received GA-loaded PVP/CEO microemulsion & were exposed to low doses of radiation. L-arginine induced a marked increase in serum lipase, amylase, and lactate dehydrogenase (LDH) activities, MYD88, TLR4 gene expression, and IL-1β. While, a significant decrease in Nrf2, Keap1, and HO-1 was observed. GA-loaded PVP/Clove oil microemulsion significantly ameliorated the above-mentioned parameters and histopathological examination.

DOI

10.21608/ejchem.2023.225404.8319

Keywords

L-arginine, PVP, Gallic acid, Microemulsion, Nrf2-Keap1-HO-1, MYD88, TLR4

Authors

First Name

Monda

Last Name

Badawy

MiddleName

-

Affiliation

Health Radiation Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

mondabadawy@gmail.com

City

Cairo

Orcid

0000-0002-9211-0009

First Name

Asmaa

Last Name

Sayed

MiddleName

-

Affiliation

Polymer Chemistry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

asmaasayedncrrt@gmail.com

City

-

Orcid

0000-0003-1882-5185

First Name

Azza

Last Name

Kayed

MiddleName

-

Affiliation

Radiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

azzakaed60@gmail.com

City

-

Orcid

-

First Name

Mai

Last Name

Mohamed

MiddleName

-

Affiliation

Polymer Chemistry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

mostafamay729@gmail.com

City

-

Orcid

-

First Name

Hebatallah

Last Name

Mohamed

MiddleName

E.

Affiliation

Radiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

hebatallahibrahim7@gmail.com

City

Cairo

Orcid

-

First Name

Ghada

Last Name

Mahmoud

MiddleName

-

Affiliation

Polymer Chemistry Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.

Email

ghadancrrt@yahoo.com

City

-

Orcid

0000-0001-8335-7766

Volume

67

Article Issue

6

Related Issue

47152

Issue Date

2024-06-01

Receive Date

2023-07-27

Publish Date

2024-06-01

Page Start

19

Page End

31

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_329510.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=329510

Order

329,510

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Gallic Acid-Loaded Microemulsion Combined with Low Doses of Radiation Ameliorates Chronic Pancreatitis in Rats via Modulation of the Nrf2-Keap1-HO-1 Pathway

Details

Type

Article

Created At

30 Dec 2024