The high demand global for food and drugs is the goal for which studies and research conducted to get safe food and medicine, so the laser light is considered a clean technology because it produces eco-friendly plants and significantly reduces using fertilizers and chemicals. Artemisia annua is one of the most important medicinal crops. The current study set out to investigate the effect of using the low-level laser (LLL) on growth, yield and essential oil, nutrients and primary metabolites of Artemisia Annua as carbohydrates and photosynthesis pigments. Pre-sowing laser irradiation of seeds results in a significant increase in vegetative parameters and active constituents, particularly with the He-Ne laser at a 10-minute exposure time, where plant height increased to 51.94 % and branch number increased to 54%. Exposure with the Blue diode laser led to an increase in total carbohydrates to 50.37% over untreated seeds, indicating nutrients were significantly improved by He-Ne laser, including calcium, which increased up to 76.35%. Physiological and biochemical changes occurred in the plant due to photochemical reactions between the laser beam and photoreceptors in the seeds of Artemisia annua.