The screening experiments from soil samples led to the isolation of some amylase-producing bacterial isolates. The bacterial isolate showed maximum amylase production was selected for further physiological and biochemical investigations. The results showed that the maximum activity (125u/ml)was obtained by using a fermentation medium consisting of (g/l) starch,10 peptone 10 yeast extract 5, ammonium sulphate 5, magnesium sulphate 0.25, CaCl2 0.25 between 80 1ml at pH 5.6,fermentation time 48 h at 300C.The obtained amylase underwent partial purification using ammonium sulphate at conc.60wt%.The purified enzyme was applied to wool fibers dyed with saffron natural dye. The phylogenic analysis of the isolated strain appeared to agree with the Bacillus mycoides. The results obtained exhibited higher color strength for the treated samples compared to the untreated fibers