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<i>Citharexylum spinosum</i> promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Organic chemistry

Abstract

Citharexylum spinosum leaves extract; n-hexane (n-Hex), n-hexane:benzene (n-Hex:Bz), chloroform (CHCl3), ethyl acetate (EtoAc), n-butanol (n-BuOH) and ethanol:H2O50% (Eth:H2O 50%) were evaluated for their anti-acetylcholinesterase, antioxidant and anti-inflammatory activities. The highest anti-acetylcholinesterase activity was obtained by n-BuOH extract followed by n-Hex:Bz and Eth:H2O 50% (IC50; 0.60, 0.80 and 1.86 µg/ml, respectively). The antioxidant results showed that, n-BuOH exhibited the highest DPPH● scavenging activity (85.91, 87.32, 56.33 and 39.43%) at different concentrations (1-100µg/µl), and 391.71±3.2μg/ml for H2O2 antioxidant method. While Eth:H2O 50% extract exhibited the highest antioxidant by reducing power method, FRAP and ABTS● scavenging activity (87.94%, 1382.29µmol of Fe2+/g sample and 20.01mmol Trolox/g sample, respectively). The highest anti-inflammatory effect obtained by n-BuOH extract followed by Eth:H2O 50% (IC50 74.50 and 109.80μg/ml, respectively). The screening of bimolecular components was carried out for n-BuOH and Eth:H2O 50% extracts by using LC-MS/MS. The LC-MS/MS profile showed the presence of organic acids; chlorogenic, gallic, caffeic, coumaric, ellagic, cinnamic, 3.4-dihydroxybenzoic, methyl gallate, syringic and ferulic) and flavonoids (rutin, vanillin, naringenin, querectin and luteolin). The current study aimed to for the first time to evaluate the anti-cholinesterase activity of C. spinosum leaves besides, the determination of the antioxidant and anti-inflammatory activities by different methods.

DOI

10.21608/ejchem.2023.235755.8594

Keywords

Citharexylum spinosum, successive extracts, cholinesterase inhibitory activity, antioxidant, Anti-inflammatory, LC-MS/MS profile

Authors

First Name

Safaa A.

Last Name

Saad

MiddleName

-

Affiliation

Natural Compounds Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt

Email

safaa_saad28@yahoo.com

City

-

Orcid

-

First Name

Howaida I.

Last Name

Abd-Alla

MiddleName

-

Affiliation

Natural Compounds Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt

Email

howaida_nrc@yahoo.com

City

-

Orcid

0000-0001-9638-8481

First Name

Hanan

Last Name

Aly

MiddleName

F.

Affiliation

Therapeutic Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt

Email

hanan_abduallah@yahoo.com

City

-

Orcid

0000-0003-3642-1571

First Name

Nagwa M. M.

Last Name

Shalaby

MiddleName

-

Affiliation

Natural Compounds Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt

Email

nagwashalaby@hotmail.com

City

Cairo

Orcid

0000-0002-6800-5460

First Name

Abd El-Moneim M. R.

Last Name

Afify

MiddleName

-

Affiliation

Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt

Email

abdelmoneimafify@yahoo.com

City

-

Orcid

-

First Name

Hanaa F. M.

Last Name

Ali

MiddleName

-

Affiliation

Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt

Email

hanaaelhelal@gmail.com

City

-

Orcid

-

First Name

Nadia

Last Name

Abdel-Moein

MiddleName

M.

Affiliation

Biochemistry Department, Faculty of Agriculture, Cairo University, Egypt

Email

dr_n_m2008@yahoo.com

City

Giza

Orcid

-

Volume

67

Article Issue

5

Related Issue

46682

Issue Date

2024-05-01

Receive Date

2023-09-12

Publish Date

2024-05-01

Page Start

63

Page End

75

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_323631.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=323631

Order

323,631

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

<i>Citharexylum spinosum</i> promotes antioxidant, anti-inflammatory, and anti-acetylcholinesterase activities

Details

Type

Article

Created At

30 Dec 2024