Currently, gamma dosimetry linked to public health is gaining more and more importance in the field of medical diagnosis. The use of different dyes, sensitive to radiation dose intensity, incorporated into different polymer biofilms has shown promising avenues for radiation dosimetry. Accordingly, the hematoxylin biomolecule as a low-ray dose sensitive dye will be integrated into a polyvinyl alcohol (PVA) polymeric host film. Direct detection of hematoxylin color change after irradiation with different g-ray doses (2, 4, 10, and 20 mGy) was apparent allowing visual estimation of radiation dose. The impact of the different radiation exposures has been evaluated via FTIR, UV, optical, colorimetric, and SEM analysis. FTIR investigation revealed an obvious chemical change with increased doses. SEM investigation showed significant morphological alteration of PVA/HX films following radiation exposure. Moreover, the produced PVA/HX films displayed good pre- and post-irradiation stability in both dark and light conditions. Furthermore, response behaviors, deduced from the different characterizations, according to increasing -ray intensities have been recorded to assess their dosimetric potential for the routine -irradiation process. Indeed, the UV-Vis response curve demonstrated a linear rise in absorbance as radiation doses increased (R = 0.992). Similarly, the different varied parameters in the colorimetry study increased linearly with increasing g-ray doses. The proposed novel dosimeter designed in nanoscale composite film might consequently offer an effective alternative for accurate and direct assessment of -ray low doses finding promising use in various medical applications.