The present study focuses on the eco-friendly synthesis of silver (Ag-NP) and zinc oxide (ZnO-NP) nanoparticles using Ficus carica leaf extract as a highly efficient reducing agent. The successful formation of (Ag-NP) and (ZnO-NP) was visually confirmed by observing the change in color or turbidity of the solutions. Additionally, characterization techniques such as UV-Vis spectroscopy, TEM, FT-IR, and XRD were employed to further investigate the synthesized nanoparticles. The UV-Vis spectrum exhibited a peak at 445, 380 nm, indicating the existence of Ag-NP and ZnO-NP, respectively. FT-IR analysis confirmed the presence of various functional groups and their corresponding bonds in the fabricated nanoparticles. Moreover, TEM analysis accurately determined the size in nanoform. The antiviral and cytotoxic effects of Ag and ZnO nanoparticles were investigated against Adenovirus, Herpes Simplex Virus type 2 (HSV-2), and Influenza Virus (H1N1). Both nanoparticle types displayed antiviral properties against both viruses. For Adenovirus, ZnO nanoparticles had a CC50 of 119.430μg/ml, while Ag nanoparticles had a CC50 of 190.18μg/ml, indicating relatively higher cytotoxicity for Zn nanoparticles. Against HSV-2, Ag nanoparticles had a lower IC50 of 22.585 μg/ml compared to ZnO nanoparticles with an IC50 of 77.745μg/ml, show casing Ag nanoparticles' higher efficacy. Similar results were observed for H1N1, where Ag nanoparticles had a lower IC50 (23.03 μg/ml) than Zn nanoparticles (33.2μg/ml). In general, Ag-NP demonstrated stronger antiviral potency with lower cytotoxicity, while ZnO-NP showed more selectivity towards H1N1.