This study aimed to identify the chemical constituents of the aqueous methanolic extract from the Lataniaverschaffeltii leaves and evaluate the biological activity. The extract was chromatographed on polyamide S6 column and eluted with H2O/MeOH mixtures of decreasing polarity, yielding seven phenolic compounds, which were identified using 1H and 13C NMR. Hepatoprotective activity of extract was evaluated for liver damage caused by CCl4 in rats by biochemical assays on serum and antioxidant enzyme as well as histopathological studies. Administration of the extract (100 and 200 mg/kg) for 21 days showed that at dose 200 mg showed significant reduction in serum ALT and AST levels to their respective standard value, significant decreases in MDA (1.21 nmol/ml) & NO (45 um/ml) and significant increases in GSH (1.05 µg / ml), TAC (1.6 µg / ml) and SOD (1.92 um/ml). Histopathological studies of liver showed moderate changes in liver histoarchitecturewhen compared to the CCl4 group . Furthermore, DPPH study revealing a promising antioxidant activity was observed (IC50 = 44.68 µg / ml) when compared to ascorbic acid.The extract of the L. verschaffeltiileaves afforded seven phenolic compounds for the first time from this species, with promising hepatoprotectiveand antioxidant activities.In conclusion, the treatment with the extract of thel. verschaffeltii leaves extract could reduce damage induced by CCl4. The mechanism of protection including the inhibition of lipid peroxidation, increasing the content of GSH, elevating the expression of antioxidant enzymes, all of which result in recuperation of biological parameters. 44.68 µg / ml) when compared to ascorbic acid.The extract of the L. verschaffeltiil eaves afforded seven phenolic compounds for the first time from this species, with promising hepatoprotective and antioxidant activities.