Glutamatergic α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors (AMPARs) are central to regulating excitatory synaptic transmission in the central nervous system (CNS) Therefore, the aim of this research is to study the biodistribution to demonstrate the concentration of [99 mTc]-nitrido-nebracetam in the target organ (brain) using healthy groups of Swiss albino mice. The radiotracer [99mTc]-nitrido-nebracetam ([99mTc]-N-Neb) complex was labelled using the core [99mTc≡N] 2+.Many factors have been studied to optimize high radiochemical yields (>97.5).Quality control studies were made perfectly. A process of biodistribution indicated that this complex, [99mTc]-N-tamsulosin, was washed out through the urinary pathway within 3 hours after injection. In addition, the brain uptake of this complex was 11.88% after 30 min post-injection as a novel probe for (AMPA) receptor targeting. Hence, the radiotracer of [99mTc]-N-Neb could be used as a radiotracer for brain imaging through the AMPA receptor.